GVIIIA (P02582) Protein Card

General Information

Name	GVIIIA
Alternative name(s)	G8a
Organism	Conus geographus (Geography cone)
Organism region	Indo-Pacific
Organism diet	piscivorous
Protein Type	Wild type
Protein precursor	GVIIIA precursor (1724)

Classification

Conopeptide class	conotoxin
Gene superfamily	S superfamily
Cysteine framework	VIII
Pharmacological family	sigma conotoxin

Sequence

GCTRTCGGOKCTGTCTCTNSSKCGCRYNVHPSG(Btr)GCG CACS(nh2)
Modified residues	position symbol name 9 O 4-Hydroxyproline 34 Btr Bromotryptophan 42 nh2 C-term amidation
Sequence evidence	protein level
Average Mass	4192.48
Monoisotopic Mass	4188.42
Isoelectric Point	12.56
Extinction Coefficient [280nm]	1490.00

Activity

IC50: 5-HT₃ receptors

Target	Organism	IC50		nhill	Agonist	Ref
5-HT3A	M. musculus	86.8 nM	[56-135]	1	Ach (100 uM)	Christensen,S.B. et al. (2015)
		53 nM	+/-3		zacopride (1 nM)	England,L.J. et al. (1998)

Ki: 5-HT₃ receptors

Target	Organism	Ki		Competitor	Agonist	Ref
5-HT3A	M. musculus	4.8 nM	+/-0.3		zacopride (1 nM)	England,L.J. et al. (1998)

References

England,L.J., Imperial,J., Jacobsen,R., Craig,A.G., Gulyas,J., Akhtar,M., Rivier,J., Julius,D. and Olivera,B.M. (1998) Inactivation of a serotonin-gated ion channel by a polypeptide toxin from marine snails Science 281:575-578

Christensen,S.B., Bandyopadhyay,P.K., Olivera,B.M. and McIntosh,J.M. (2015) αS-conotoxin GVIIIB potently and selectively blocks α9α10 nicotinic acetylcholine receptors Biochem. Pharmacol. 96:349-356

Internal links

Protein Precursor	GVIIIA precursor (1724)
Nucleic acids
Structure

External links

Tools

Please cite:
Kaas Q, Yu R, Jin AH, Dutertre S and Craik DJ. ConoServer: updated content, knowledge, and discovery tools in the conopeptide database. Nucleic Acids Research (2012) 40(Database issue):D325-30
Kaas Q, Westermann JC, Halai R, Wang CK and Craik DJ. ConoServer, a database for conopeptide sequences and structures. Bioinformatics (2008) 24(3):445-6

ConoServer is managed at the Institute of Molecular Bioscience IMB, Brisbane, Australia.

The database and computational tools found on this website may be used for academic research only, provided that it is referred to ConoServer, the database of conotoxins (http://www.conoserver.org) and the above reference is cited. For any other use please contact David Craik (d.craik@imb.uq.edu.au).

Contacts:
David Craik
Quentin Kaas

Last updated: Monday 5 June 2023