GVIIIB (P08941) Protein Card

General Information
Name GVIIIB
Organism Conus geographus (Geography cone)
Organism region Indo-Pacific
Organism diet piscivorous
Protein Type Wild type
Protein precursor GVIIIB precursor (8942)
Notes Christensen et al.,2015 reported that GVIIIB is over 100-fold selective for the α9α10 nAChR compared to other nAChR subtypes, including α1β1δε,5-HT3 serotonin receptor,α3β2,α6/α3β2β3,α3β4,α4β2 and α7.

Classification
Conopeptide class conotoxin
Gene superfamily S superfamily
Cysteine framework VIII
Pharmacological family alpha conotoxin

Sequence
SGSTCTCFTSTNCQGSCECLSPPGCYCSNNGIRQRGCSCTC
PGT(nh2)
Modified residues
positionsymbolname
45nh2C-term amidation
Sequence evidence protein level
Average Mass 4464.89
Monoisotopic Mass 4461.63
Isoelectric Point 10.72
Extinction Coefficient [280nm] 1490.00

Activity

IC50: Nicotinic acetylcholine receptors

TargetOrganismIC50nhillAgonistRef
α9α10R. norvegicus9.8 nM[6.6-14.4]1.32Ach (10 uM)Christensen,S.B. et al. (2015)

Percentage inhibition: Nicotinic acetylcholine receptors

TargetOrganism% inhibitionConcentrationAgonistRef
α3β2R. norvegicus4+/-3100nMAch (100 uM)Christensen,S.B. et al. (2015)
α3β4R. norvegicus0100nMAch (100 uM)Christensen,S.B. et al. (2015)
α4β2R. norvegicus5+/-5100nMAch (100 uM)Christensen,S.B. et al. (2015)
α6/α3β2β3R. norvegicus11+/-8100nMAch (100 uM)Christensen,S.B. et al. (2015)
α7R. norvegicus3+/-4100nMAch (100 uM)Christensen,S.B. et al. (2015)

References
Christensen,S.B., Bandyopadhyay,P.K., Olivera,B.M. and McIntosh,J.M. (2015) αS-conotoxin GVIIIB potently and selectively blocks α9α10 nicotinic acetylcholine receptors Biochem. Pharmacol. 96:349-356

Internal links
Protein Precursor GVIIIB precursor (8942)
Nucleic acids
Structure

External links

Tools