GVIIJ covalent dimer (P06955) Protein Card

General Information
Name GVIIJ covalent dimer
Alternative name(s) (GVIIJ)2
Organism synthetic construct
Protein Type Synthetic
Parent GVIIJ
Notes This peptide was synthesised as a dimer, which is linked by a disulfide bond at C24. Protein mass indicated in this entry should be multiplied by 2 to reflect this.

Classification
Conopeptide class conotoxin
Gene superfamily
Pharmacological family mu conotoxin

Sequence
GWCGDOGATCGKLRLYCCSGFCDCYTKTCKDKSSA
Modified residues
positionsymbolname
6O4-Hydroxyproline
Sequence evidence protein level
Average Mass 3739.22
Monoisotopic Mass 3736.50
Isoelectric Point 10.10
Extinction Coefficient [280nm] 8605.00

Activity

Kd: Voltage-Gated Sodium Channels

TargetOrganismKdRef
Nav1.2R. norvegicus(Expressed in X. laevis oocytes. Kon & Koff rate constants recorded +/- SD in original article.)0.065 μMZhang,M.M. et al. (2015)
Nav1.6M. musculus(Expressed in X. laevis oocytes. Kon & Koff rate constants recorded +/- SD in original article.)1.038 μMZhang,M.M. et al. (2015)

References
Zhang,M.M., Gajewiak,J., Azam,L., Bulaj,G., Olivera,B.M. and Yoshikami,D. (2015) Probing the Redox States of Sodium Channel Cysteines at the Binding Site of μO§-Conotoxin GVIIJ. Biochemistry 54:3911-3920

Internal links
Nucleic acids
Structure

External links

Tools