LtXIVA (P02588) Protein Card

General Information
Name LtXIVA
Alternative name(s) Lt033,Lt14.1,Lt14a,lt14a
Organism Conus litteratus
Organism region Indo-Pacific
Organism diet vermivorous
Protein Type Wild type
Protein precursor LtXIVA precursor (27)
Notes

LtXIVa displayed analgesic activity in a hot plate assay (Sun et al. 2011).

Analgesia was also observed against second-phase pain in formalin-induced inflammatory pain model, and in a paw withdrawal threshold test of mechanical pain (Ren et al. 2015).

Effects downstream of nAChR: inhibits Ca(2+) influx, ERK1/2 phosphorylation and c-fos/NOS expression (Ren et al. 2015).

LtXIVA treatment did induce a morphine-dependent phenotype in mice, and genes associated with drug dependence were not up-regulated (Ren et al. 2015). LtXIVA treatment reversed morphine withdrawal symptoms in mice [Ren et al. 2015].

Wu et al. (2021) studied the pharmacological and toxicological properties of LtXIVa. The study showed that 1, 5 and 10 mg/kg doses of LtXIVa could prolong the pentobarbital-induced sleep time of mice.

Classification
Conopeptide class conotoxin
Gene superfamily L superfamily
Cysteine framework XIV
Pharmacological family alpha conotoxin

Sequence
MCPPLCKPSCTNC(nh2)
Modified residues
positionsymbolname
14nh2C-term amidation
Sequence evidence nucleic acid level
Average Mass 1391.71
Monoisotopic Mass 1390.53
Isoelectric Point 10.17
Extinction Coefficient [280nm] NA

Synthetic variants
Lt14a variantMRPPLCKPSCTNC
LtXIVA [K7A]MCPPLCAPSCTNC(nh2)

References
Peng,C., Tang,S., Pi,C., Liu,J., Wang,F., Wang,L., Zhou,W. and Xu,A. (2006) Discovery of a novel class of conotoxin from Conus litteratus, lt14a, with a unique cysteine pattern Peptides 27:2174-2181
Sun,D., Ren,Z., Zeng,X., You,Y., Pan,W., Zhou,M., Wang,L. and Xu,A. (2011) Structure-function relationship of conotoxin lt14a, a potential analgesic with low cytotoxicity. Peptides 32:300-305
Ren,Z., Wang,L., Qin,M., You,Y., Pan,W., Zhou,L., Sun,D. and Xu,A. (2015) Pharmacological characterization of conotoxin lt14a as a potent non-addictive analgesic. Toxicon 96:57-67
Zhang, H., Fu, Y., Wang, L., Liang, A., Chen, S. and Xu, A. (2019) Identifying novel conopepetides from the venom ducts of Conus litteratus through integrating transcriptomics and proteomics Journal of proteomics 192:346-357
Li,X., Chen,W., Zhangsun,D. and Luo,S. (2020) Diversity of Conopeptides and Their Precursor Genes of Conus Litteratus. Mar Drugs 18
Wu Y, Qiang Y, Zhang G, Zhou M. (2021) Acute toxicity and micronucleus test of conotoxin lt14a in mice. Basic Clin Pharmacol Toxicol 129:52-60

Internal links
Protein Precursor LtXIVA precursor (27)
Nucleic acids
Structure Solution structure of Lt14a

External links
Ncbi Q2I2R5

Tools