VxXXB (P01685) Protein Card

General Information
Name VxXXB
Alternative name(s) Vx20.2,VxXIIB
Organism Conus vexillum
Organism region Indo-Pacific
Organism diet vermivorous
Protein Type Wild type
Protein precursor VxXXB precursor (3631)
Vx99 precursor (7037)
Vx101 precursor (7038)
Vx102 precursor (7039)
Vx109 precursor (7040)
Vx130 precursor (7041)
Vx132 precursor (7042)
Vx135 precursor (7043)
Vx141 precursor (7044)
Vx142 precursor (7045)
Vx154 precursor (7046)
Vx163 precursor (7047)
Vx164 precursor (7048)
Vx184 precursor (7049)
Notes B>Framework: This framework was assigned to be XII on discovery of VxXXA , VxXXB and VxXXC (previously named VxXIIA, VxXIIB and VxXIIC respectively) 2006, which also had been used for conotoxins from the I superfamily (Brown et al. 2005). Loughnan et al. now propose framework designation XX (see reference).

Dimers: Mature D superfamily peptides form hetero-, homo- and pseudohomodimers (identical sequence, different posttranslational modifications). Entries for mature peptides in ConoServer are for monomers.

The swiss-prot card describes positions 14, 30 and 49 as being hydroxyprolines and position 49 as amidated C-terminus which is in contradiction with Loughnan et al 2006.

Classification
Conopeptide class conotoxin
Gene superfamily D superfamily
Cysteine framework XX
Pharmacological family alpha conotoxin

Sequence
DD(Gla)S(Gla)CIINTRDSPWGRCCRTRMCGSMCCPRNG
CTCVYHWRRGHGCSCPG
Modified residues
positionsymbolname
3GlaGamma carboxylic glutamic acid
5GlaGamma carboxylic glutamic acid
Sequence evidence protein level
Average Mass 5735.35
Monoisotopic Mass 5731.18
Isoelectric Point 11.73
Extinction Coefficient [280nm] 12490.00

Activity

IC50: Nicotinic acetylcholine receptors

TargetOrganismIC50nhillAgonistRef
α1β1γδR. norvegicus3.5mM+/-0.80.991uM AchLoughnan,M. et al. (2006)
α3β2R. norvegicus8.4nM+/-1.80.76100uM AchLoughnan,M. et al. (2006)
α4β2R. norvegicus228nM+/-840.68100uM AchLoughnan,M. et al. (2006)
α7R. norvegicus400pM+/-2001.8100uM nicotineLoughnan,M. et al. (2006)

References
Loughnan,M., Nicke,A., Jones,A., Schroeder,C.I., Nevin,S.T., Adams,D.J., Alewood,P.F. and Lewis,R.J. (2006) Identification of a novel class of nicotinic receptor antagonists: dimeric conotoxins VxXIIA, VxXIIB, and VxXIIC from Conus vexillum J. Biol. Chem. 281:24745-24755
Loughnan,M.L., Nicke,A., Lawrence,N. and Lewis,R.J. (2009) Novel alphaD-conopeptides and their precursors identified by cDNA cloning define the D-conotoxin superfamily. Biochemistry 48:3717-3729
Prashanth,J.R., Dutertre,S., Jin,A.H., Lavergne,V., Hamilton,B., Cardoso,F.C., Griffin,J., Venter,D.J., Alewood,P.F. and Lewis,R.J. (2016) The role of defensive ecological interactions in the evolution of conotoxins. Mol. Ecol. 25:598-615

Internal links
Protein Precursor VxXXB precursor (3631)
Vx99 precursor (7037)
Vx101 precursor (7038)
Vx102 precursor (7039)
Vx109 precursor (7040)
Vx130 precursor (7041)
Vx132 precursor (7042)
Vx135 precursor (7043)
Vx141 precursor (7044)
Vx142 precursor (7045)
Vx154 precursor (7046)
Vx163 precursor (7047)
Vx164 precursor (7048)
Vx184 precursor (7049)
Nucleic acids
Structure

External links
UniProtKB/Swiss-Prot P0C1W6
Ncbi P0C1W6

Tools