PIIIE (P01611) Protein Card

General Information

Name	PIIIE
Alternative name(s)	P3.8,P3e
Organism	Conus purpurascens
Organism region	Eastern Pacific
Organism diet	piscivorous
Protein Type	Wild type
Protein precursor	PIIIE precursor (1406)

Classification

Conopeptide class	conotoxin
Gene superfamily	M superfamily
Cysteine framework	III
Pharmacological family	alpha conotoxin

Sequence

HOOCCLYGKCRRYOGCSSASCCQR(nh2)
Modified residues	position symbol name 2 O 4-Hydroxyproline 3 O 4-Hydroxyproline 14 O 4-Hydroxyproline 25 nh2 C-term amidation
Sequence evidence	protein level
Average Mass	2717.09
Monoisotopic Mass	2715.08
Isoelectric Point	12.81
Extinction Coefficient [280nm]	2980.00

Activity

IC50: Nicotinic acetylcholine receptors

Target	Organism	IC50		Agonist	Ref
α1β1δε	M. musculus	7.4uM		100uM Ach	Lluisma,A.O. et al. (2008)
α1β1γδ	M. musculus	>10uM		100uM Ach	Lluisma,A.O. et al. (2008)
	T. marmorata	177nM	+/-3	1uM Ach	Van Wagoner,R.M. et al. (2003)

Synthetic variants

PIIIE [K9S]	HOOCCLYGKCRRYOGCSSASCCQR(nh2)
PIIIE [R12O,Y13F]	HOOCCLYGKCROFOGCSSASCCQR(nh2)
PIIIE [S17Y,S18N,S20L]	HOOCCLYGKCRRYOGCSSASCCQR(nh2)

References

Shon,K.J., Grilley,M., Jacobsen,R., Cartier,G.E., Hopkins,C., Gray,W.R., Watkins,M., Hillyard,D.R., Rivier,J., Torres,J., Yoshikami,D. and Olivera,B.M. (1997) A noncompetitive peptide inhibitor of the nicotinic acetylcholine receptor from Conus purpurascens venom Biochemistry 36:9581-9587

Mitchell,S.S., Shon,K.J., Foster,M.P., Davis,D.R., Olivera,B.M. and Ireland,C.M. (1998) Three-dimensional solution structure of conotoxin psi-PIIIE, an acetylcholine gated ion channel antagonist Biochemistry 37:1215-1220

Van Wagoner,R.M. and Ireland,C.M. (2003) An improved solution structure for psi-conotoxin PiiiE. Biochemistry 42:6347-6352

Himaya, S.W.A., Mari, F. and Lewis, R.J. (2018) Accelerated proteomic visualization of individual predatory venoms of Conus purpurascens reveals separately evolved predation-evoked venom cabals Scientific reports 8:1-8

Internal links

Protein Precursor	PIIIE precursor (1406)
Nucleic acids
Structure	SOLUTION STRUCTURE OF CONOTOXIN Y-PIIIE FROM CONUS PURPURASCENS, NMR, 14 STRUCTURES SOLUTION STRUCTURE OF THE NONCOMPETITIVE SKELETAL MUSCLE NICOTINIC ACETYLCHOLINE RECEPTOR ANTAGONIST PSI-CONOTOXIN PIIIE

External links

Ncbi	1AS5_A, 1JLO_A

Tools

Please cite:
Kaas Q, Yu R, Jin AH, Dutertre S and Craik DJ. ConoServer: updated content, knowledge, and discovery tools in the conopeptide database. Nucleic Acids Research (2012) 40(Database issue):D325-30
Kaas Q, Westermann JC, Halai R, Wang CK and Craik DJ. ConoServer, a database for conopeptide sequences and structures. Bioinformatics (2008) 24(3):445-6

ConoServer is managed at the Institute of Molecular Bioscience IMB, Brisbane, Australia.

The database and computational tools found on this website may be used for academic research only, provided that it is referred to ConoServer, the database of conotoxins (http://www.conoserver.org) and the above reference is cited. For any other use please contact David Craik (d.craik@imb.uq.edu.au).

Contacts:
David Craik
Quentin Kaas

Last updated: Saturday 23 September 2023