RVIIIA (P01574) Protein Card

General Information

Name	RVIIIA
Alternative name(s)	R8a
Organism	Conus radiatus
Organism region	Indo-Pacific
Organism diet	piscivorous
Protein Type	Wild type
Protein precursor	RVIIIA precursor (4000)
Notes	RVIIIA has no activity against the glutamate-activated chloride channels.

Classification

Conopeptide class	conotoxin
Gene superfamily	S superfamily
Cysteine framework	VIII
Pharmacological family	alpha conotoxin

Sequence

KCNFDKCKGTGVYNCG(Gla)SCSC(Gla)GLHSCRCTYNI GSMKSGCACICTYY
Modified residues	position symbol name 17 Gla Gamma carboxylic glutamic acid 22 Gla Gamma carboxylic glutamic acid
Sequence evidence	protein level
Average Mass	5170.75
Monoisotopic Mass	5166.93
Isoelectric Point	10.64
Extinction Coefficient [280nm]	5960.00

Activity

IC50: Nicotinic acetylcholine receptors

Target	Organism	IC50		nhill	Agonist	Ref
α1β1δε	H. sapiens	100nM			1uM Ach	Teichert,R.W. and Jimenez,E.C. and Olivera,B.M. (2005)
	R. norvegicus	43.1 nM	[17-108]	0.71	Ach (100 uM)	Christensen,S.B. et al. (2015)
α1β1γδ	H. sapiens	33nM			2uM Ach	Teichert,R.W. and Jimenez,E.C. and Olivera,B.M. (2005)
α9α10	R. norvegicus	187 nM	[138-255]	1.9	Ach (10 uM)	Christensen,S.B. et al. (2015)

Percentage inhibition: 5-HT₃ receptors

Target	Organism	% inhibition		Concentration	Agonist	Ref
5-HT3A	H. sapiens	0		10uM		Teichert,R.W. and Jimenez,E.C. and Olivera,B.M. (2005)

Percentage inhibition: Nicotinic acetylcholine receptors

Target	Organism	% inhibition		Concentration	Agonist	Ref
α3β2	H. sapiens	65		10uM	100uM Ach	Teichert,R.W. and Jimenez,E.C. and Olivera,B.M. (2005)
	R. norvegicus	50		10uM	100uM Ach	Teichert,R.W. and Jimenez,E.C. and Olivera,B.M. (2005)
α3β4	H. sapiens	20		10uM	100uM Ach	Teichert,R.W. and Jimenez,E.C. and Olivera,B.M. (2005)
α4β2	H. sapiens	20		10uM	100uM Ach	Teichert,R.W. and Jimenez,E.C. and Olivera,B.M. (2005)
α6/α3β2β3	R. norvegicus	50		10uM	100uM Ach	Teichert,R.W. and Jimenez,E.C. and Olivera,B.M. (2005)
α7	H. sapiens	90		10uM	100uM Ach	Teichert,R.W. and Jimenez,E.C. and Olivera,B.M. (2005)
		70		1uM	100uM Ach	Teichert,R.W. and Jimenez,E.C. and Olivera,B.M. (2005)

References

Teichert,R.W., Jimenez,E.C. and Olivera,B.M. (2005) Alpha S-conotoxin RVIIIA: a structurally unique conotoxin that broadly targets nicotinic acetylcholine receptors Biochemistry 44:7897-7902

Christensen,S.B., Bandyopadhyay,P.K., Olivera,B.M. and McIntosh,J.M. (2015) αS-conotoxin GVIIIB potently and selectively blocks α9α10 nicotinic acetylcholine receptors Biochem. Pharmacol. 96:349-356

Internal links

Protein Precursor	RVIIIA precursor (4000)
Nucleic acids
Structure

External links

UniProtKB/Swiss-Prot	P0C1W3
Ncbi	P0C1W3

Tools

Please cite:
Kaas Q, Yu R, Jin AH, Dutertre S and Craik DJ. ConoServer: updated content, knowledge, and discovery tools in the conopeptide database. Nucleic Acids Research (2012) 40(Database issue):D325-30
Kaas Q, Westermann JC, Halai R, Wang CK and Craik DJ. ConoServer, a database for conopeptide sequences and structures. Bioinformatics (2008) 24(3):445-6

ConoServer is managed at the Institute of Molecular Bioscience IMB, Brisbane, Australia.

The database and computational tools found on this website may be used for academic research only, provided that it is referred to ConoServer, the database of conotoxins (http://www.conoserver.org) and the above reference is cited. For any other use please contact David Craik (d.craik@imb.uq.edu.au).

Contacts:
David Craik
Quentin Kaas

Last updated: Monday 4 December 2023